p53 Steady-State Level

Legend : P53 Steady-State Level

3-D models of human ribosomal subunits based on PDB entries 3J3D, 3J3A, 3J3F, and 3J3B. R-proteins are color-coded according to p53 steady-state accumulation. Left, subunit interface views; right, solvent exposed views. The aminoacyl (A), peptidyl (P) and exit (E) tRNA sites are indicated. Morphological features of the subunits are highlighted. On the LSU: the L1-stalk, the central protuberance (CP) and the phospho-stalk (P-stalk). On the SSU, the beak (Be), head (H), platform (Pt), body (Bd), lef foot (Lf) and right foot (Rf).

The histogram shows r-proteins classified according to their impact on p53 steady-state accumulation. The nontargeting (Scr) control is shown in red, the RPS in green, and the RPL in magenta. The histogram bars are the means of triplicates with SD indicated. R-proteins whose depletion leads to a 5-fold increase in p53 levels are highlighted in a shaded box.

 steady201504

 

Protein

Average

  • 0.5
  • 0.5
  • 0.5
  • 0.5
  • 0.6
  • 0.6
  • 0.7
  • 0.8
  • 0.8
  • 0.8
  • 0.8
  • 0.9
  • 0.9
  • 0.9
  • 0.9
  • 1.0
  • 1.0
  • 1.0
  • 1.0
  • 1.0
  • 1.1
  • 1.1
  • 1.1
  • 1.2
  • 1.2
  • 1.3
  • 1.3
  • 1.3
  • 1.4
  • 1.4
  • 1.4
  • 1.5
  • 1.5
  • 1.6
  • 1.7
  • 1.7
  • 1.8
  • 1.8
  • 1.9
  • 1.9
  • 2.0
  • 2.1
  • 2.1
  • 2.2
  • 2.2
  • 2.2
  • 2.4
  • 2.5
  • 2.9
  • 3.2
  • 3.2
  • 3.3
  • 3.5
  • 4.2
  • 4.2
  • 4.6
  • 4.8
  • 4.8
  • 5.0
  • 5.1
  • 5.2
  • 5.4
  • 5.5
  • 5.7
  • 5.7
  • 6.2
  • 6.3
  • 6.3
  • 6.9
  • 6.9
  • 7.1
  • 7.2
  • 7.2
  • 8.0
  • 8.2
  • 8.3
  • 8.3
  • 8.5
  • 8.9
  • 8.9
  • 10.0
  • 10.4

Stdev

  • 0.1
  • 0.1
  • 0.1
  • 0.0
  • 0.1
  • 0.1
  • 0.2
  • 0.3
  • 0.2
  • 0.1
  • 0.0
  • 0.1
  • 0.1
  • 0.2
  • 0.2
  • 0.1
  • 0.4
  • 0.2
  • 0.3
  • 0.3
  • 0.2
  • 0.1
  • 0.1
  • 0.1
  • 0.4
  • 0.3
  • 0.2
  • 0.1
  • 0.2
  • 0.2
  • 0.1
  • 0.8
  • 0.4
  • 1.0
  • 0.3
  • 0.7
  • 1.5
  • 0.5
  • 0.5
  • 0.6
  • 0.2
  • 0.3
  • 0.3
  • 0.6
  • 0.8
  • 0.2
  • 0.7
  • 0.5
  • 0.7
  • 0.1
  • 0.5
  • 0.3
  • 0.1
  • 0.7
  • 0.8
  • 0.8
  • 0.6
  • 2.0
  • 2.0
  • 0.6
  • 0.4
  • 0.6
  • 1.3
  • 0.6
  • 2.1
  • 2.4
  • 0.7
  • 2.9
  • 1.1
  • 0.9
  • 2.6
  • 1.8
  • 0.7
  • 2.5
  • 2.2
  • 1.4
  • 1.2
  • 0.3
  • 1.1
  • 0.5
  • 0.2
  • 2.5